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VOLUME 7 , ISSUE 2 ( July-Dec, 2018 ) > List of Articles


Assessment of qualitative defects in patients with normal sperm counts

Abilash Sasidharannair Chandrakumari, Dost Mohamed Khan

Keywords : Sperm count, Sperm morphology, Hematoxylin stain

Citation Information : Chandrakumari AS, Khan DM. Assessment of qualitative defects in patients with normal sperm counts. 2018; 7 (2):32-35.

DOI: 10.5005/jp-journals-10085-7305

License: CC BY-NC 4.0

Published Online: 30-05-2020

Copyright Statement:  Copyright © 2018; The Author(s).


Background: Assessment of sperm morphology is an important criterion in determining the semen quality and it plays a major role in male infertility. Aim: To assess the morphological defects of the spermatozoa in patients with normal sperm counts. Materials and Methods: This was a cross-sectional study carried out in a tertiary care centre for a period of one year. A total of sixty semen samples with normal sperm counts were included. Morphological assessments of spermatozoa were performed on smears stained with Hematoxylin and Eosin and Papanicolaou.stains. The defects were categorized based on the abnormalities in different parts such as head, middle piece and tail. Results: All cases 60 samples despite normal sperm count showed morphological defects. The percentage of defects ranged from 1% to 20% but none of the sample showed abnormality more than 20%. The most common defect observed was in the region of head (tapering head - 86.67%) followed by thick middle piece defect (71.67%). Ninety five percentages of cases showed multiple defects. Conclusions: Qualitative defects are observed in all patients with normal sperm counts

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  1. Anafarta K, Beduk Y, Arikan N. Principles of Urology. Ankara, Sun Publishing. 2007;18:122-4.
  2. Agarwal A, Mulgund A, Hamada A, Chyatte MR. A unique view on male infertility around the globe. Reprod Biol Endocrinol 2015;13:37.
  3. Vasan SS. Semen analysis and sperm function tests: How much to test? Indian J Urol 2011;27:41.8.
  4. World Health Organization. Standard procedures. In: Cooper TG, editor. WHO laboratory manual for the examination and processing of human semen. 5th ed. Geneva: World Health Organization; 2010. p:7.10.
  5. Auger J. Assessing human sperm morphology: Top models, underdogs or biometrics? Asian J Androl 2010;12:36.46.
  6. Lingappa HA, Govindashetty AM, Krishnamurthy A, Puttaveerachary AK, Manchaiah S, Shimoga IC, et al. Quest for an ideal, simple and costeffective stain for morphological assessment of sperms. J Clin Diagn Res. 2015;9(10):1-4
  7. Singh S, Sharma S, Jain M, Chauhan R. Importance of Papanicolaou staining for sperm morphologic analysis: Comparison with an automated sperm quality analyzer. Am J Clin Pathol 2011;136:247.51.
  8. Menkveld R. Clinical significance of the low normal sperm morphology value as proposed in the fifth edition of the WHO laboratory manual for the examination and processing of human semen. Asian J Androl 2010;12:47.58.
  9. Karabulut A, Tekin A. Alterations in the morphology and motility of spermatozoa: Relation with total sperm count. Pam Med J 2013;6:1.4.
  10. Andrade. Rocha FT. Sperm parameters in men with suspected infertility. Sperm characteristics, strict criteria sperm morphology analysis and hypoosmotic swelling test. J Reprod Med 2001;46:577.82.
  11. Nikolettos N, Kupker W, Demirel C, Schopper B, Blasig C, Sturm R, et al. Fertilization potential of spermatozoa with abnormal morphology. Hum Reprod 1999;14(Suppl 1):47.70.
  12. Ta.demir I, Ta.demir M, Tavukcuoglu S, Kahraman S, K. Effect of abnormal sperm head morphology on the outcome of intracytoplasmic sperm injection in humans. Hum Reprod 1997;12:1214.7.
  13. Cao X, Cui Y, Zhang X, Lou J, Zhou J, Wei R, et al. The correlation of sperm morphology with unexplained recurrent spontaneous abortion: A systematic review and meta.analysis. Oncotarget 2017;8:55646.56.
  14. Auger J, Eustache F, Andersen AG, Irvine DS, Jorgensen N, Skakkebaek NE, et al. Sperm morphological defects related to environment, lifestyle and medical history of 1001 male partners of pregnant women from four European cities. Hum Reprod 2001;16:2710.7.
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